@conference {167,
	title = {Production of Methylripariochromene-A Bioactive Compound   at Eupatorium riparium Reg. by In Vitro Tissue Culture Technique },
	booktitle = {Seminar Nasional ke-22 Perhimpunan Biologi Indonesia},
	year = {2013},
	publisher = {Fakultas Biologi Unsoed},
	organization = {Fakultas Biologi Unsoed},
	address = {Purwokerto, Jawa Tengah, Indonesia},
	abstract = {Eupatorium riparium Reg.  have been reported that contain of methylripariochromene-A (MRC-A) and this compounds can be used to treat hypertension, anticancer, diuretic, decrease of sistolic/diastolic, and also were used in inhibiting colony of fungus and weeds. MRC-A can be produced by callus tissue culture with short and continuous production cycles. In general, high levels auxin (2,4-D) has been reported that  to be suitable for triggering callus induction in tissue culture.  This research aimed is to determine suitable combination of plant growth regulator (PGR) namely cytokinin (kinetin) and auxin (2,4D) and also to know  concentration level of MRC-A production at  E. riparium callus culture. The Method of this research was  tissue culture  technique with combination of cytokinin and auxin were 1ppm kinetin  and 2 ppm 2,4-D; 1 ppm kinetin and 3 ppm 2,4-D; 1 ppm kinetin and 4 ppm 2,4-D and combination of 1 ppm kinetin and 5 ppm 2,4-D,  and medium for callus induction using Murrashige-Skoog (MS). MRC-A concentration analysed using TLC-Densitometry. The result of study, based on statistic analysis showed that callus dried weight of E. riparium treatment with combination of cytokinin and auxin  were for 1 ppm kinetin and 2 ppm 2,4-D (0.092 g); 1 ppm kinetin and 4 ppm 2,4-D (0.069 g); 1 ppm kinetin and 5 ppm 2,4-D (0.039 g) and 1 ppm kinetin  and 3 ppm 2,4-D (0.037 g) and its significantly different between treatment. Methylripariochromene-A concentration of callus        E. riparium was higher than MRC-A from leaves of    E. riparium. The highest concentration of MRC-A base on analysed by TLC-Densitometry founded on callus 1 ppm kinetin and 2 ppm 2,4-D (0.98\%), others 1 ppm kinetin and 4 ppm 2,4-D (0.77\%); 1 ppm kinetin and 5 ppm 2,4-D (0.45\%); 1 ppm kinetin and 3 ppm 2,4-D (0.44\%) and leaf of E. riparium (0.42\%), respectively. The best combination of PGR in callus culture to produced MRC-A was 1 ppm kinetin and 2 ppm 2,4-D. This research suggested that MRC-A bioactive compound can be produced by tissue culture in vitro was added trigger using elicitors and precursors},
	keywords = {4-D), auxin (2, cytokinin (kinetin), Eupatorium riparium Reg., Methylripariochromene-A, TLC-Densitometry},
	author = {Linus Yhani Chrystomo and Aditya Krishar Karim and Issirep Sumardi and L. Hartanto Nugroho and Subagus Wahyuono}
}
