@conference {97,
	title = {The Relative Expression Of Brlf1 Epstein-Barr Virus : A Potensial Use For  The Biomarker Of Tumour Progresivity In Nasopharyngeal Carcinoma },
	booktitle = {Seminar Nasional ke-22 Perhimpunan Biologi Indonesia},
	year = {2013},
	publisher = {Fakultas Biologi Unsoed},
	organization = {Fakultas Biologi Unsoed},
	address = {Purwokerto, Jawa Tengah, Indonesia},
	abstract = {In South-East Asian region, the NPC incidence is the highest in worldwide, i.e. Malaysia, Indonesia and Singapore. Indonesian population has high ethnic diversity with a population of about 225 million with NPC incidency rate of 6.2 per 100,000 population per year. EBV  infection is not found in all normal cell of nasopharyngeal epithelium, however it can be found in all NPC cells. Protein BRLF1-EBV (Rta) and protein BZLF1-EBV (Zta) act a main transcription activator in EBV infection, particularly in immediate-early lytic phase of  EBV lytic cycle. Expression of mRNA BRLF1 EBV specifically associated with NPC tissue tumor patient. This study aims to investigate a potential use for relative  expression of mRNA  BRLF1 EBV as a biomarker of NPC tumour progressivity.  The research was done from Januari 2009 till Januari 2012. This reseach design was cross sectional study. The subjects of research are patient who have been diagnosed of having NPC. The sample used in this research is NPC biopsy tumour tissue from Departement ENT, Dr. Cipto Mangunkusumo Hospital - Faculty of Medicine Universitas Indonesia. All research subject were given informed consent before taking part in this research.  Total of  research subject were 67 people which we consist of 2 people for NPC WHO-1, 4 people for NPC WHO-2, 46 people NPC WHO-3 patients and 15 healthy people for control.  The expression of mRNA LMP1 and that of mRNA BRLF1 in patient NPC WHO-3 was determined by RT-PCR technique. The relative expression of mRNA BRLF1 in patient NPC WHO-3 expressed mRNA LMP1 EBV was determined by RT-qPCR technique and calculated using 2-ΔΔCt. The positivity of expression of mRNA LMP1 was 42/46 samples (93,1 percent) and that of expression of mRNA BRLF1 was 30/46 samples (65,2 percent). The median relative expression of mRNA BRLF1 on status M1 (after distant metastases) has signficantly increased (p = 0,005**) than on status M0 (before distant metastasis). The median relative expression of mRNA BRLF1 in advanced stadium in NPC distant metastases (stadium IV-C) has signficantly increased  (p = 0,007**) than in advanced NPC  before distant metastases (stadium III, IV-A, IV-B). The result indicate that the relative expression of mRNA },
	author = {Daniel Joko Wahyono and Zanil Musa and Lisnawati and Dwi Anita Suryandari and Bambang Hermani and Purnomo Soeharso}
}
@conference {96,
	title = {Hubungan Polimorfisme Gen Tcrβ  Dan Tcrγ Dengan Penyakit Karsinoma Nasofaring Pada Populasi Indoensia},
	booktitle = {Seminar Nasional ke-22 Perhimpunan Biologi Indonesia},
	year = {2013},
	publisher = {Fakultas Biologi Unsoed},
	organization = {Fakultas Biologi Unsoed},
	address = {Purwokerto, Jawa Tengah, Indonesia},
	abstract = {Karsinoma Nasofaring (KNF) adalah penyakit yang bersifat multifaktor dan  endemik secara geografis di dunia. Populasi Indonesia mempunyai insidensi KNF cukup tinggi yaitu 6,2/100.000 penduduk per tahun. Faktor genetika penjamu merupakan salah satu faktor risiko timbulnya penyakit KNF. Polimorfisme genetik adalah faktor genetika yang dapat menentukan suseptibilitas pejamu terhadap KNF. Polimorfisme gen TCRβ dan gen TCRγ merupakan faktor genetika penjamu yang berkaitan dengan suseptibilitas terhadap penyakit KNF yang berkaitan dengan ras tertentu. Penelitian ini bertujuan mengetahui apakah polimorfisme gen TCRβ dan gen TCRγ pada penjamu berasosiasi dengan suseptibilitas terhadap penyakit karsinoma nasofaring. Desain penelitian ini adalah studi cross sectional. Distribusi genotip dan alotip TCRβ ditentukan dengan teknik PCR-RFLP. Distribusi genotip dan alotip TCRγ ditentukan dengan teknik PCR-RFLP dan nested Long-PCR. Distribusi genotip dan alotip TCRβ pada kontrol tidak berbeda dengan penderita KNF. Distribusi genotip dan alotip TCRγ pada kontrol tidak berbeda dengan penderita KNF. Hasil penelitian ini menunjukkan bahwa alotip TCRβ dan alotip TCRγ tertentu tidak menentukan suseptibilitas terhadap KNF},
	keywords = {penyakit KNF, polimorfisme gen TCRβ, polimorfisme gen TCRγ},
	author = {Daniel Joko Wahyono and Zanil Musa and Lisnawati Lisnawati and Dwi Anita Suryandari and Bambang Hermani and Purnomo Soeharso}
}
